The Netherlands international suffered the injury during Barcelona's training session ahead of their La Liga game against Villarreal. Initial tests suggest a muscle tear that could keep him out for four weeks.
De Jong's absence is a significant blow for Hansi Flick, who had just regained a fully fit squad. The midfielder will miss key matches including the Copa del Rey semi-final, Champions League Round of 16, and several La Liga fixtures, as well as the upcoming international break with Netherlands.