Goals from Luca Ranieri, Rolando Mandragora, and Roberto Piccoli secured the win for Fiorentina, who now have one foot in the Conference League last 16. The Italian side traveled to Bialystok without several key players including injured David De Gea, Moise Kean, Dodo, Manor Solomon and Albert Gudmundsson.
Jagiellonia were missing suspended top scorer Afimico Pululu and midfielder Taras Romanczuk, with the second leg scheduled for February 26. Luca Lezzerini made his second UEFA appearance in goal for Fiorentina, who dominated proceedings despite the absences.